Oxygen enhancement ratios of cancer cells after exposure to intensity modulated x-ray fields: DNA damage and cell survival

Abstract Hypoxic cancer cells within solid tumours show radio-resistance, leading to malignant progression in fractionated radiotherapy. When prescribing dose under heterogeneous oxygen pressure with intensity-modulated radiation fields, intercellular signalling could have an impact on radiosensitivity between in-field and out-of-field (OF) cells. However, the of hypoxia radio-sensitivity modulated intensity remains be fully clarified. Here, we investigate OF radio-sensitivities using two types cells, DU145 H1299. Using a nBIONIX hypoxic culture kit shielding technique irradiate 50% cell flask, enhancement ratios for double-strand breaks (DSB) death endpoints were determined. These vitro measurements indicate that impacts although survival dose-dependent smaller compared those uniformly irradiated decreased shown as consistent tendency both DSB endpoints, suggesting radiation-induced communication is importance advanced radiotherapy dose-distributions such